- Sudbury Cyclones
- Alliance United (L2M)
- North Toronto Nitros (L2W)
- Whitby FC (L2M)
- Woodridge Strikers (L2W)
- Alliance United FC (L2W)
- Sudbury Cyclones (L2W)
- Guelph United FC
- Whitby FC
- Sudbury Cyclones (L2M)
- Alliance United FC
- Darby FC
- FC London
- Master’s FA
- Master’s FA (L2M)
- Master’s FA (L2W)
- North Mississauga SC
- North Mississauga SC (L2W)
- Pickering FC (L2M)
- Scrosoppi (L2W)
- Simcoe County Rovers FC
- Simcoe County Rovers FC (L2M)
- Simcoe County Rovers FC (L2W)
- The Borough (L2M)
- The Borough FC
- Unionville Milliken SC
- Unionville Milliken SC (L2M)
- Unionville Milliken SC (L2W)
- Vaughan Azzurri
- Vaughan Azzurri (L2M)
- Vaughan Azzurri (L2W)
- Waterloo United
- Whitby FC (L2W)
- Windsor City FC
- Pickering FC
- Pickering FC (L2W)
- York United Academy
- Hamilton United